Abstract
This
research explained the effect of exogenous sucrose on the levels of DPPH free
radical scavenging ability, total phenolics and vitamin C as well as the L-galactono-1,4-lactone
dehydrogenase (GalLDH), superoxide
dismutase (SOD), catalase (CAT) and
ascorbate peroxidase (APX) activitiesof soybean (Glycine max L.) sprouts. Soybean seeds were
soaked in the various solutions prepared with 0, 1, 10 and 100 mM sucrose at 25°C for 12 hr and then
sprayed with these solutions every 12 hr during the germination for 5 days. The higher
concentration of exogenous sucrose decreased the L* value of soybean sprouts; whereas, the levels of DPPH free radical scavenging
ability, total phenolic, vitamin C and GalLDH, SOD, CAT and APXactivities increased. The soybean sprouts
from 100mM sucrose-treated seeds showed the maximum
levels ofDPPH free
radical scavenging ability, total phenolics, vitamin C and GalLDH, SOD, CAT and APXactivities, i.e. 85%,
164.95 mg GAE g-1 FW, 120.65 mg 100g-1 FW, 25.32 U g-1FW,
12.00U g-1FW,
25.36 U g-1FW and 924 U g-1FW, respectively. Thus, it
suggested that the sucrose treatment helped to promote the soybean sprouts in containing
high-levels of antioxidant activities.
Keywords: Soybean sprouts, Sucrose,
SOD, CAT, APX